@article {19832, title = {A soft 3D polyacrylate hydrogel recapitulates the cartilage niche and allows growth-factor free tissue engineering of human articular cartilage}, journal = {Acta Biomaterialia}, volume = {19}, year = {2019}, month = {2019-03-22 00:00:00}, pages = {1-11}, publisher = {Elsevier}, edition = {1}, abstract = {

Cartilage degeneration or damage treatment is still a challenge, but, tissue engineering strategies, which combine cell therapy strategies, which combine cell therapy and scaffolds, and have emerged as a promising new approach. In this regard,\ polyurethanes\ and\ polyacrylates\ polymers have been shown to have clinical potential to treat osteochondral injuries. Here, we have used polymer\ microarraystechnology to screen 380 different polyurethanes and polyacrylates polymers. The top polymers with potential to maintain\ chondrocyte\ viability were selected, with\ scale-up\ studies performed to evaluate their ability to support chondrocyte proliferation during long-term culture, while maintaining their characteristic phenotype. Among the selected polymers, poly (methylmethacrylate-co-methacrylic acid), showed the highest level of chondrogenic potential and was used to create a 3D\ hydrogel. Ultrastructural morphology,\ microstructure\ and\ mechanical testing\ of this novel hydrogel revealed robust characteristics to support chondrocyte growth. Furthermore,\ in vitro\ and\ in vivo\ biological assays demonstrated that chondrocytes cultured on the hydrogel had the capacity to produce extracellular matrix similar to hyaline cartilage, as shown by increased expression of\ collagen type II,\ aggrecan\ and Sox9, and the reduced expression of the fibrotic marker{\textquoteright}s\ collagen type I.\ In conclusion, hydrogels generated from poly (methylmethacrylate-co-methacrylic acid) created the appropriate niche for chondrocyte growth and phenotype maintenance and might be an optimal candidate for cartilage tissue-engineering applications.

}, keywords = {cartilage tissue engineering, hydrogel, Poly (methylmethacrylate-co-methacrylic acid), Polyacrylate, Polymer microarray}, issn = {1742-7061}, doi = {10.1016/j.actbio.2019.03.040}, url = {https://www.sciencedirect.com/science/article/pii/S1742706119302156}, author = {Jim{\'e}nez, G. and Venkateswaran, S. and L{\'o}pez-Ruiz, E. and Per{\'a}n, M. and Pernagallo, S. and D{\'\i}az-Monch{\'o}n, J. J. and Canadas, R. F. and Antich, C. and Oliveira, J. M. and Callanan, A. and Walllace, R. and Reis, R. L. and Monta{\~n}ez, E. and Carrillo, E. and Bradley, M. and Marchal, J. A.}, editor = {Wagner, W. R.} }