@article {19735, title = {Ultrasound-assisted biosynthesis of novel methotrexate-conjugates}, journal = {Ultrasonics Sonochemistry}, volume = {48}, year = {2018}, month = {2018-05-17 00:00:00}, pages = {51-56}, publisher = {Elsevier}, abstract = {

New methotrexate-acylglycerols and methotrexate-cyclodextrins (α, β and γ-CD) conjugates were obtained via esterification or transesterification reactions. All reactions were catalysed by esterases namely immobilized Lipase from\ Candida antarctica B\ and Lipase from\ Thermomyces lanuginosus. The use of ultrasound to assist the reactions revealed to be a key factor to obtain high conversion yields on both MTX conjugates. Transesterification reactions including long chain triacylglycerols were only successful when ultrasound was applied. In cyclodextrins esterification a higher number of MTX molecules was also linked to cyclodextrins when ultrasound was used. All the conjugates were characterized by MALDI-TOF and NMR spectroscopy.

}, keywords = {Biosynthesis, cyclodextrins, Lipases, Methotrexate, Triacylglycerols, Ultrasound}, issn = {1350-4177}, doi = {10.1016/j.ultsonch.2018.05.017}, url = {https://www.sciencedirect.com/science/article/pii/S1350417718302682?via\%3Dihub}, author = {Noro, J. and Reis, R. L. and Cavaco-Paulo, A. and Silva, C.} }