@article {19631, title = {Silk fibroin/amniotic membrane 3D bi-layered artificial skin}, journal = {Biomedical Materials}, volume = {13}, year = {2018}, month = {2018-02-20 00:00:00}, pages = {035003}, publisher = {IOP}, abstract = {

Burn injuries have been reported to be an important cause of morbidity and mortality and they are still considered as unmet clinical need. Although there is a myriad of effective stem cells that have been suggested for\ skin\ regeneration, there is no one ideal scaffold. The aim of this study was to develop a three-dimensional (3D) bi-layer scaffold made of biological decellularized human\ amnioticmembrane\ (AM) with viscoelastic electrospun nanofibrous\ silkfibroin\ (ESF) spun on top. The fabricated\ 3D\ bi-layer AM/ESF scaffold was submerged in ethanol to induce β-sheet transformation as well as to get a tightly coated and inseparable bi-layer. The biomechanical and biological properties of the\ 3D\ bi-layer AM/ESF scaffold were investigated. The results indicate significantly improved mechanical properties of the AM/ESF compared with the AM alone. Both the AM and AM/ESF possess a variety of suitable adhesion cells without detectable cytotoxicity against adipose tissue-derived mesenchymal stem cells (AT-MSCs). The AT-MSCs show increased expression of two main pro-angiogenesis factors, vascular endothelial growth factor and basic fibroblast growth factor, when cultured on the AM/ESF for 7 days, when comparing with AM alone. The results suggest that the AM/ESF scaffold with autologous AT-MSCs has excellent cell adhesion and proliferation along with production of growth factors which serves as a possible application in a clinical setting for\ skin\ regeneration.

}, keywords = {human amniotic membrane, regenerative medicine, Scaffold, Silk Fibroin, skin}, issn = {1748-6041}, doi = {10.1088/1748-605X/aa999b}, url = {http://iopscience.iop.org/article/10.1088/1748-605X/aa999b/meta}, author = {Gholipourmalekabadi, M. and Samadikuchaksaraei, A. and Seifalian, A. M. and Urbanska, A. M. and Ghanbarian, H. and Hardy, J. G. and Omrani, M. D. and Mozafari, M. and Reis, R. L. and Kundu, S. C.} }