@article {19624, title = {Vascularized bone tissue engineered system comprising autologous growth differentiation factors and mesenchymal stem cells}, journal = {2018 TERMIS World Congress}, year = {2018}, month = {2018-09-04 00:00:00}, address = {Kyoto, Japan}, abstract = {

Reconstruction of large bone defects still remaining a major clinical orthopedic challenge, since repair of a bone defect is not only the process of new bone formation, but also the formation of new blood vessels - angiogenesis [1]. Bone morphogenetic proteins (BMPs) and vascular endothelial growth factor (VEGF) are involved on cell differentiation and bone vascularization to construct viable osseous tissue [2, 3]. Therefore, herein is hypothesized that the synergistic effect of autologous BMP-2 and VEGF parallel bound over a single nanofibrous substrate (NFMs) can lead to a successful osteogenic and angiogenic differentiation of mesenchymal stem cells. To achieve such ambitious goal, an engineered system was developed comprising BMP-2 and VEGF antibodies immobilized over the same structure in side-by-side fashion, trying to recreate the vasculature of a bone tissue. Furthermore, immobilized antibodies were capable of selectively immobilize the respective growth factor from a biological fluid (i.e. platelet lysate). The osteogenic and angiogenic potential of this engineered biofunctionalized system was assessed by culturing human bone marrow mesenchymal stem cells (hBM-MSCs) during 21 days without exogenous induction. Bare NFMs cultured with hBM-MSCs under basal and standard osteogenic or angiogenic differentiation media were used as negative and positive controls, respectively. Biological data demonstrate that the engineered biofunctional NFM comprising BMP-2 is able to promote osteogenesis, whereas the VEGF is able to promote angiogenesis of hBM-MSCs. Moreover, the vascularized bone tissue engineered system proposed is able to promote spatial angiogenesis and osteogenesis of hBM-MSCs, targeting an effective vascularized bone tissue engineering approach.

[1]S. Almubarak, et al., Bone, 2016;

[2]D. Barati, et al., J Control Release 223, 2016;

[3]M. Bouyer, et al., Biomaterials, 2016.

}, keywords = {mesenchymal stem cells, Nanofibrous substrate, Vascularized bone tissue engineered}, url = {https://www2.convention.co.jp/termis-wc2018/pdf/s_poster3.pdf}, author = {Casanova, M. R. and Fernandes, E. M. and Reis, R. L. and Martins, A. and Neves, N. M.} }