@article {19507,
	title = {Fish sarcoplasmic proteins as a high value marine material for wound dressing applications},
	journal = {Colloids and Surfaces B: Biointerfaces},
	volume = {167},
	year = {2018},
	month = {2018-07-01 00:00:00},
	pages = {310-317},
	publisher = {Elsevier},
	abstract = {<p>Fish sarcoplasmic proteins (FSP) constitute around 25{\textendash}30\% of the total fish muscle protein. As the FSP are water soluble, FSP were isolated from fresh cod <em>(Gadus morhua) </em>by centrifugation. By SDS-PAGE was possibly determined the composition of FSP extracts (FSP-E). The FSP-E denature at 44.12 {\textpm} 2.34oC, as characterized by differential scanning calorimetry thermograms (DSC). The secondary structure of FSP-E is mainly composed by α-helix structure, as determined by circular dichroism. The cytocompatibility of FSP-E, at concentrations ranging from 5 to 20 mg/mL, was also investigated. For concentrations lower than 10 mg/mL, no cytotoxicity was observed over 72h of fibroblasts culture. Further on, FSP membranes (FSP-M) were produced by spin coating technique to evaluate its properties. FSP-M had uniform surface as analyzed by SEM, and the amount of α-helix structure increased when compared with the FSP-E. The FSP-M are more stable than the FSP-E, since they presented a denaturation temperature of 58.88 {\textpm} 3.36oC, according to the DSC thermogram. FSP-M shown distinctive mechanical properties, with a stiffness of 16.57 {\textpm} 3.95 MPa and a yield strength of 23.85 {\textpm} 5.97 MPa. Human lung fibroblasts (MRC-5 cell line) cultured in direct contact with FSP-M demonstrate to be cytocompatible for 48h. Based on these results, FSP can be considered a potential biomaterial, recovered from the nature, for wound dressing applications.</p>},
	keywords = {Cytocompatibility, membranes, Physico-chemical characterization, Sarcoplasmic proteins, Spin coating},
	issn = {0927-7765},
	doi = {10.1016/j.colsurfb.2018.04.002},
	url = {https://www.sciencedirect.com/science/article/pii/S0927776518302066},
	author = {Vieira, S. F. and Franco, A. R. and Fernandes, E. M. and Amorim, S. and Ferreira, H. and Pires, R. A. and Reis, R. L. and Martins, A. and Neves, N. M.}
}
