@article {19478, title = {From honeycomb- to microsphere-patterned surfaces of poly(lactic acid) and a starch-poly(lactic acid) blend via the breath figure method}, journal = {Journal of Applied Biomaterials \& Functional Materials}, volume = {15}, year = {2017}, month = {2017-01-26 00:00:00}, pages = {e31-e42}, publisher = {Wichtig Publishing}, abstract = {

Background: This study investigated the preparation of ordered patterned surfaces and/or microspheres from a natural-based polymer, using the breath figure and reverse breath figure methods.
Methods: Poly(D,L-lactic acid) and starch poly(lactic acid) solutions were precipitated in different conditions {\textendash} namely, polymer concentration, vapor atmosphere temperature and substrate {\textendash} to evaluate the effect of these conditions on the morphology of the precipitates obtained.

Results: The possibility of fine-tuning the properties of the final patterns simply by changing the vapor atmo- sphere was also demonstrated here using a range of compositions of the vapor phase. Porous films or discrete particles are formed when the differences in surface tension determine the ability of polymer solution to sur- round water droplets or methanol to surround polymer droplets, respectively. In vitro cytotoxicity was assessed applying a simple standard protocol to evaluate the possibility to use these materials in biomedical applications. Moreover, fluorescent microscopy images showed a good interaction of cells with the material, which were able to adhere on the patterned surfaces after 24 hours in culture.

Conclusions: The development of patterned surfaces using the breath figure method was tested in this work for the preparation of both poly(lactic acid) and a blend containing starch and poly(lactic acid). The potential of these films to be used in the biomedical area was confirmed by a preliminary cytotoxicity test and by morphological observation of cell adhesion.

}, keywords = {Biomaterials, Breath figures, natural polymers, Patterns, Poly(lactic acid), Starch}, issn = {2280-8000}, doi = {10.5301/jabfm.5000281}, url = {http://www.jab-fm.com/article/f68d88a8-f5fa-49f5-8b3e-0d6cdbebee02}, author = {Duarte, A. R. C. and Maniglio, D. and Sousa, N. and Mano, J. F. and Reis, R. L. and Migliaresi, C.} }