@article {19111, title = {Sulfated alginate as a mimic of sulfated glycosaminoglycans: Binding of growth factors and effect on stem cell behavior}, journal = {Advanced Biosystems}, volume = {1}, year = {2017}, month = {2017-06-16 00:00:00}, pages = {1700043}, publisher = {Wiley}, abstract = {

Sulfated glycosaminoglycans (GAGs) are principal elements of the extracellular\ matrix, where they are involved in a plethora of signaling pathways mainly via\ interactions with diverse proteins such as growth factors and cytokines. However,\ the mechanisms that drive these interactions are not yet clear, mostly
because of the difficulty to access large quantities of homogeneously sulfated\ natural GAGs. In this work, GAG mimics are synthesized from readily available\ alginate with different degrees of sulfation (DS, from 0.8 to 2.6) by simple\ process. The effect of the DS is determined on the binding of basic fibroblast\ growth factor (FGF-2). The enzyme-linked immunosorbent assay demonstrates\ that the binding of FGF-2 is significantly greater for alginates with high\ DS as compared to unmodified and low sulfated analogs. These results are
further applied to engineer FGF-2-loaded substrates for stem cell culturing\ using the layer-by-layer approach. All films support the attachment and growth\ of adipose derived stem cells (ADSCs). Noteworthy, highly sulfated alginates\ maintain the stemness of the ADSCs that exhibit remarkably long filopodia.\ These results can be exploited in the engineering of novel substrates that\ induce targeted cell behavior via controlled protein delivery and for tissue engineering\ constructs applicable in various regenerative approaches.

}, keywords = {ALGINATE, biomimetic materials, growth factors, layer-by-layer, sulfated glycosaminoglycans}, issn = {2366-7478}, doi = {10.1002/adbi.201700043}, url = {http://onlinelibrary.wiley.com/doi/10.1002/adbi.201700043/epdf}, author = {Mhanna, R. and Becher, J. and Schnabelrauch, M. and Reis, R. L. and Pashkuleva, I.} }