@article {19057,
	title = {Extraction and characterization of collagen from Antarctic and Sub-Antarctic squid and its potential application in hybrid scaffolds for tissue engineering},
	journal = {Materials Science \& Engineering C-materials For Biological Applications},
	volume = {78},
	year = {2017},
	month = {2017-04-21 00:00:00},
	publisher = {Elsevier},
	abstract = {<p>Collagen is the most abundant protein found in mammals and it exhibits a low immunogenicity, high biocompatibility and biodegradability when compared with others natural polymers. For this reason, it has been explored for the development of biologically instructive biomaterials with applications for tissue substitution and regeneration. Marine origin collagen has been pursued as an alternative to the more common bovine and porcine origins. This study focused on squid (Teuthoidea: Cephalopoda), particularly the Antarctic squid Kondakovia longimana and the Sub-Antarctic squid Illex argentinus as potential collagen sources. In this study, collagen has been isolated fromthe skins of the squids using acid-based and pepsin-based protocols, with the higher yield being obtained from I. argentinus in the presence of pepsin. The produced collagen has been characterized in terms of physicochemical properties, evidencing an amino acid profile similar to the one of calf collagen, but exhibiting a less preserved structure, with hydrolyzed portions and a lower melting temperature. Pepsin-soluble collagen isolated fromI. argentinus was selected for further evaluation of biomedical potential, exploring its incorporation on poly-ε-caprolactone (PCL) 3D printed scaffolds for the development of hybrid scaffolds for tissue engineering, exhibiting hierarchical features.</p>},
	keywords = {3D printing, Antarctic, hierarchical scaffolds, marine biomaterials, Squid collagen, Three dimensional hybrid scaffolds},
	issn = {0928-4931},
	doi = {10.1016/j.msec.2017.04.122},
	author = {Coelho, R. C. G. and Marques, A. L. P. and Oliveira, S. M. and Diogo, G. S. and Pirraco, R. P. and Moreira-Silva, J. and Xavier, J. C. and Reis, R. L. and Silva, T. H. and Mano, J. F.}
}
