@article {19006, title = {Osteogenesis evaluation of duck{\textquoteright}s feet derived collagen/hydroxyapatite sponges immersed in dexamethasone}, journal = {Biomaterials Research}, volume = {21}, year = {2017}, month = {2017-01-02 00:00:00}, pages = {1-7}, publisher = {BioMed Central}, abstract = {

Background: The aim of this study was to investigate the osteogenesis effects of DC and DC/HAp sponge immersed in without and with dexamethasone.

Methods: The experimental groups in this study were DC and DC/HAp sponge immersed in without dexamethasone (Dex(-)DC and Dex(-)-DC/HAp group) and with dexamethasone (Dex(+)-DC and Dex(+)-DC/HAp group). We characterized DC and DC/HAp sponge using compressive strength, scanning electron microscopy (SEM). Also, osteogenic differentiation of BMSCs on sponge (Dex(-)DC, Dex(-)-DC/HAp, Dex(+)-DC and Dex(+)-DC/HAp group) was assessed by SEM, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide (MTT) assay, alkaline phosphatase (ALP) activity assay and reverse transcription-PCR (RT-PCR).

Results: In this study, we assessed osteogenic differentiation of BMSCs on Duck{\textquoteright}s feet-derived collagen (DC)/ HAp sponge immersed with dexamethasone Dex(+)-DC/HAp. These results showed that Dex(+)-DC/HAp group increased cell proliferation and osteogenic differentiation of BMSCs during 28 days.

Conclusion: From these results, Dex(+)-DC/HAp can be envisioned as a potential biomaterial for bone regeneration applications.

}, keywords = {bone, Collagen, hydroxyapatite}, issn = {2055-7124}, doi = {10.1186/s40824-017-0088-4}, author = {Kook, Y. J. and Lee, D. H. and Song, J. E. and Tripathy, N. and Jeon, Y. S. and Oliveira, J. M. and Reis, R. L. and Khang, G.} }