@article {19005,
	title = {Depth (Z-axis) control of cell morphologies on micropatterned surfaces},
	journal = {Journal Of Bioactive And Compatible Polymers},
	volume = {30},
	year = {2015},
	month = {2015-04-19 00:00:00},
	pages = {1-13},
	publisher = {SAGE},
	abstract = {<p><span>In this study, cell responses on micropatterned films that were changing in groove{\textendash}ridge widths and pattern depth were investigated to compare the degree of size effects from X{\textendash}Y and Z planes. Poly(caprolactone) films with five different groove{\textendash}ridge sizes and three pattern depths were prepared by hot embossing technique. In general, the morphologies of osteoblast cell were not changed noticeably by the size changes in groove{\textendash}ridges with the same depth size. However, cell morphologies were changed significantly when pattern depths were increased from 1.35 to 4.95 {\textmu}m. Also, the cell morphology change between different groove{\textendash}ridges was significant when the pattern depth was small (1.35 {\textmu}m), and these effects were diminished or masked when the pattern depth was increased to 4.95 {\textmu}m. Linear regression analysis further clarifies that unit size changes in depth may affect cell length and orientation rates 2.4 and 4 times, respectively, in comparison to rates obtained from X{\textendash}Y planes.</span></p>},
	keywords = {cell guiding, cell morphology, lithography, micropatterning, pattern depth},
	issn = {08839115},
	doi = {10.1177/0883911515580354},
	url = {http://journals.sagepub.com/doi/pdf/10.1177/0883911515580354},
	author = {Baran, E. T. and Pirraco, R. P. and Cerqueira, M. T. and Marques, A. P. and Retolaza, A. and Merino, S. and Neves, N. M. and Reis, R. L.}
}
