@article {18972, title = {By-products of Scyliorhinus canicula, Prionace glauca and Raja clavata: A valuable source of predominantly 6S sulfated chondroitin sulfate}, journal = {Carbohydrate Polymers}, volume = {157}, year = {2017}, month = {2017-02-10 00:00:00}, pages = {31-38}, publisher = {Elsevier}, abstract = {

Chondroitin sulfate (CS) was isolated from Scyliorhinus canicula (fin, head and skeleton), Prionace glauca (head), and Raja clavata (skeleton) by-products from fish processing industry using environmentally friendly processes. The molecular weight was determined by gel permeation chromatography and the sugar composition and sulfation position by NMR and SAX-HPLC after enzymatic digestion. The CSs showed a prevalent 6S GalNAc sulfation for the 3 species (4S/6S ratio lower than 1). A higher 6S sulfation was observed for P. glauca head and R. clavata skeleton (4S/6S ratio below 0.20) than for S. canicula (4S/6S ratio ca. 0.6). The existence of CS samples with such low 4S/6S ratio has only been observed before in a rare species of shark (Mitsukutina owatoni, globin shark). The good extraction yields achieved make S. canicula, P. glauca and R. clavata fish industry by-products a useful source of 6-sulfated chondroitin sulfate.

}, keywords = {Chondoritin sulfate, Fishery, NMR; GPC}, issn = {0144-8617}, doi = {10.1016/j.carbpol.2016.09.050}, url = {http://dx.doi.org/10.1016/j.carbpol.2016.09.050}, author = {Novoa-Carballal, R. and P{\'e}rez-Mart{\'\i}n, R.; Blanco, S. M.; and Nunes, C. and Coimbra, M. A. and Silva, T. H. and Reis, R. L. and Jos{\'e}, V. A.}, editor = {Elsevier} }