@article {18809, title = {Reinforcement of Poly-L-lactic acid Electrospun Membranes with Strontium Borosilicate Bioactive Glasses for Bone Tissue Engineering}, journal = {Acta Biomaterialia}, volume = {44}, year = {2016}, month = {2016-10-15 00:00:00}, pages = {168-177}, publisher = {Elsevier}, abstract = {

Herein, for the first time, we combined poly-L-lactic acid (PLLA) with a strontium borosilicate bioactive glass (BBG-Sr) using electrospinning to fabricate a composite bioactive PLLA membrane loaded with 10\% (w/w) of BBG-Sr glass par- ticles (PLLA-BBG-Sr). The composites were characterised by scanning electron microscopy (SEM) and microcomputer tomography (μ-CT), and the results showed that we successfully fabricated smooth and uniform fibres (1{\textendash}3 μm in width) with a homogeneous distribution of BBG-Sr microparticles (\<45 μm). Degradation studies (in phosphate buffered saline) demonstrated that the incorporation of BBG-Sr glass particles into the PLLA membranes increased their degradability and water uptake with a continuous release of cations. The addition of BBG-Sr glass particles enhanced the membrane{\textquoteright}s mechanical properties (69\% higher Young modulus and 36\% higher tensile strength). Furthermore, cellular in vitro evaluation using bone marrow-derived mesenchymal stem cells (BM-MSCs) demonstrated that PLLA-BBG-Sr mem- branes promoted the osteogenic differentiation of the cells as demonstrated by increased alkaline phosphatase activity and up-regulated osteogenic gene expression (Alpl, Sp7 and Bglap) in relation to PLLA alone. These results strongly suggest that the composite PLLA membranes reinforced with the BBG-Sr glass particles have potential as an effective biomater- ial capable of promoting bone regeneration.\ 

}, keywords = {BM-MSCs, Mineralisation, osteogenic differentiation, PLLA membranes, Strontium borosilicate glasses}, issn = {1742-7061}, doi = {10.1016/j.actbio.2016.08.042}, url = {http://www.sciencedirect.com/science/article/pii/S174270611630438X}, author = {Fernandes, J. S. and Gentile, P. and Martins, I. M. and Neves, N. M. and Miller, C. and Crawford, A. and Pires, R. A. and Hatton, P. V. and Reis, R. L.} }