@inproceedings {18697,
	title = {Investigation of Dendrimer-based nanoparticles cellular uptake and cell tracking in a semi-automated microfluidic platform},
	journal = {Frontiers},
	year = {2016},
	month = {2016-03-30 00:00:00},
	address = {Montreal, Canada},
	abstract = {<p>A microfluidic device such as Kima Pump and Vena8 biochip is able to realize functions that are not easily imaginable in conventional biological analysis, such as highly parallel, sophisticated high-throughput analysis and single-cell analysis in a well-defined manner\&nbsp;<sup>[1]</sup>.</p>
<p>Cancer cell tracking within the microfluidic model will be achieved by grafting fluorescent label probe Fluorescein-5(6)-isothiocyanate (FITC) to dendrimer nanoparticles allowing cell visualization by immunofluorescent staining followed by fluorescence microscopy. In this study, synthesis and physicochemical characterization of Carboxymethyl-chitosan/poly(amidoamine) dendrimer nanoparticles (CMCht/PAMAM NP{\textquoteright}s) were performed<sup>[2]</sup>. \&nbsp;Several cancer cell lines such as a HeLA (cervical carcinoma cell line), HTTC-116 (Colon Carcinoma) and Glioblastome cell line (GBM) were exposed to different concentrations of CMCht/PAMAM dendrimer nanoparticles over a period of 7d. After finding the adequate NP concentration, the internalization efficiency was tested, as well as cellular trafficking, in static and dynamic conditions (Kima Pump bioreactor).</p>},
	keywords = {Cancer, Microfluidic, Nanoparticles},
	issn = {2296-4185},
	doi = {10.3389},
	url = {http://www.frontiersin.org/10.3389/conf.FBIOE.2016.01.02034/event_abstract},
	author = {Carvalho, M. R. and Maia, F. R. and Reis, R. L. and Oliveira, J. M.}
}
