@article {18643, title = {Xenogeneic Modulation of Macrophages and Dendritic Cells by Undifferentiated and Differentiated Adipose Tissue-derived Stem Cells}, journal = {TERMStem 2014}, year = {2014}, month = {2014-10-22 00:00:00}, address = {Porto, Portugal}, abstract = {

Adult mesenchymal stem cells (MSCs) are considered to be "immunologically privileged" and have been widely studied as tools in the field of tissue engineering and regenerative medicine (TERM) as well as in immunotherapeutic approaches. In a previous work when human adipose tissue-derived stem cells (hASCs) subcutaneously implanted in micewe did not identify an adverse host response1. In this study we hypothesised that undifferentiated hASCs and derived osteoblasts and chondrocytes are unable to activate murine bone marrow-derived macrophages (mBMM{\O}s) and dendritic cells (mBMDCs) into M2 phenotype, aiding tissue regeneration.

Murine BMM{\O}s or mBMDCs were plated in direct contact with undifferentiated and osteo or chondro-differentiated hASCs for 4h, 10h, 24h and 72h. The cytokine profile was analysed by qRT-PCR and the surface markers were detected by flow cytometry. The direct interaction of both cell types was observed by time lapse microscopy.

The results showed that murine BMM{\O}s and BMDCs activate in similarly after contacting tissue culture polystyrene or after contacting both undifferentiated and osteo or chondro-differentiated hASCs. This was confirmed by the expression of IL-1, IL-10, IL-4, TNF-α and IFN-γ (genetic profile) and surface markers (CD206++, CD336++, MHC II+ and CD86++) detection.

These data suggest immunotolerance exerted by osteo- and chondro-differentiated hASCs in xenogeneic approaches. It is therefore possible to consider the potential of hASCs in contemporary xenogenic tissue engineering and regenerative medicine strategies, as well as host immune system modulation in autoimmune diseases.

Acknowledgments: RL3-TECT-NORTE-01-0124-FEDER-000020, co-financed by North Portugal

Regional Operational Program (ON.2-O Novo Norte), under the National Strategic Reference Framework, through the European Regional Development Fund

1-Santos, TC et al., Tissue Eng A, 19(7-8), 83, 2013\ 

Adult mesenchymal stem cells (MSCs) are considered to be "immunologically privileged" and have been widely studied as tools in the field of tissue engineering and regenerative medicine (TERM) as well as in immunotherapeutic approaches. In a previous work when human adipose tissue-derived stem cells (hASCs) subcutaneously implanted in micewe did not identify an adverse host response1. In this study we hypothesised that undifferentiated hASCs and derived osteoblasts and chondrocytes are unable to activate murine bone marrow-derived macrophages (mBMM{\O}s) and dendritic cells (mBMDCs) into M2 phenotype, aiding tissue regeneration.

Murine BMM{\O}s or mBMDCs were plated in direct contact with undifferentiated and osteo or chondro-differentiated hASCs for 4h, 10h, 24h and 72h. The cytokine profile was analysed by qRT-PCR and the surface markers were detected by flow cytometry. The direct interaction of both cell types was observed by time lapse microscopy.

The results showed that murine BMM{\O}s and BMDCs activate in similarly after contacting tissue culture polystyrene or after contacting both undifferentiated and osteo or chondro-differentiated hASCs. This was confirmed by the expression of IL-1, IL-10, IL-4, TNF-α and IFN-γ (genetic profile) and surface markers (CD206++, CD336++, MHC II+ and CD86++) detection.

These data suggest immunotolerance exerted by osteo- and chondro-differentiated hASCs in xenogeneic approaches. It is therefore possible to consider the potential of hASCs in contemporary xenogenic tissue engineering and regenerative medicine strategies, as well as host immune system modulation in autoimmune diseases.

Acknowledgments: RL3-TECT-NORTE-01-0124-FEDER-000020, co-financed by North Portugal

Regional Operational Program (ON.2-O Novo Norte), under the National Strategic Reference Framework, through the European Regional Development Fund

1-Santos, TC et al., Tissue Eng A, 19(7-8), 83, 2013\ 

}, keywords = {Human Adipose tissue-derived stem cells, Murine Dendritic Cells, Murine Macrophages, Xenogeneic Immunomodulation}, author = {Santos, T. C. and Rodrigues, D. B. and Cerqueira, M. T. and Pirraco, R. P. and Marques, A. P. and Reis, R. L.} }