@article {18628, title = {Osteogenic differentiation of human mesenchymal stem cells in the absence of osteogenic supplements: a surface-roughness gradient study.}, journal = {Acta Biomaterialia}, volume = {28}, year = {2015}, month = {2015-10-09 00:00:00}, pages = {64-75}, publisher = {Elsevier}, abstract = {

The use of biomaterials to direct osteogenic differentiation of human mesenchymal stem cells (hMSCs) in

the absence of osteogenic supplements is thought to be part of the next generation of orthopedic

implants. We previously engineered surface-roughness gradients of average roughness (Ra) varying from

the sub-micron to the micrometer range (0.5{\textendash}4.7 μm), and mean distance between peaks (RSm) gradually

varying from 214 μm to 33 μm. Here we have screened the ability of such surface-gradients of

polycaprolactone to influence the expression of alkaline phosphatase (ALP), collagen type 1 (COL1) and

mineralization by hMSCs cultured in dexamethasone (Dex)-deprived osteogenic induction medium

(OIM) and in basal growth medium (BGM). Ra 1.53 μm/RSm 79 μm in Dex-deprived OI medium,

and Ra 0.93 μm/RSm 135 μm in BGM consistently showed higher effectiveness at supporting the

expression of the osteogenic markers ALP, COL1 and mineralization, compared to the tissue culture polystyrene

(TCP) control in complete OIM. The superior effectiveness of specific surface-roughness revealed

that this strategy may be used as a compelling alternative to soluble osteogenic inducers in orthopedic

applications featuring the clinically relevant biodegradable polymer polycaprolactone.

}, keywords = {Biomaterials, High-throughput screening (gradient), mesenchymal stem cells, Microstructures, osteogenesis}, issn = {1742-7061}, doi = {10.1016/j.actbio.2015.09.028}, url = {http://www.sciencedirect.com/science/article/pii/S1742706115301215}, author = {Faia-Torres, A. B. and Charnley, M. and Goren, T. and Guimond-Lischer, S. and Rottmar, M. and Maniura-Weber, K. and Spencer, N. D. and Reis, R. L. and Textor, M. and Neves, N. M.} }