@article {18400, title = {Investigation of Dendrimer-based nanoparticles cellular uptake and cell tracking in a semi-automated microfluidic platform}, booktitle = {27th European Conference on Biomaterials ESB2015, Krakow, Poland}, year = {2015}, month = {2015-08-30 00:00:00}, address = {Poland}, abstract = {

A microfluidic device such as Kima Pump and Vena8 biochip is able to realize functions that are not easily imaginable in conventional biological analysis, such as highly parallel, sophisticated high-throughput analysis and single-cell analysis in a well-defined manner [1].

Cancer cell tracking within the microfluidic model will be achieved by grafting fluorescent label probe Fluorescein-5(6)-isothiocyanate (FITC) to dendrimer nanoparticles allowing cell visualization by immunofluorescent staining followed by fluorescence microscopy. In this study, synthesis and physicochemical characterization of Carboxymethyl-chitosan/poly(amidoamine) dendrimer nanoparticles (CMCht/PAMAM NP{\textquoteright}s) were performed[2].\  Several cancer cell lines such as a HeLA (cervical carcinoma cell line), HTTC-116 (Colon Carcinoma) and Glioblastome cell line (GBM) were exposed to different concentrations of CMCht/PAMAM dendrimer nanoparticles over a period of 7d. After finding the adequate NP concentration, the internalization efficiency was tested, as well as cellular trafficking, in static and dynamic conditions (Kima Pump bioreactor).

}, keywords = {Cancer, Microfluidics}, author = {Carvalho, M. R. and Maia, F. R. and Reis, R. L. and Oliveira, J. M.} }