@article {18354,
	title = {Compartmentalized Bioencapsulated Liquefied 3D Macro-Construct by Perfusion-Based Layer-by-Layer Technique.},
	journal = { RSC Advances},
	volume = {5},
	year = {2014},
	month = {2014-11-14 00:00:00},
	pages = {2511-2516},
	publisher = {ROYAL SOC CHEMISTRY, THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND},
	abstract = {<p>Self-supporting, millimeter length 3D constructs consisting of individualized liquefied compartments, were produced using cell encapsulated hydrogel beads as building blocks. A perfusion-based layer-by-layer approach was used that allowed bioencapsulated beads to assemble, pattern, hold and attach to produce non-liquefied 3D constructs with controlled shape, displaying the binding feature of a continuous nanometric multilayer coating. No binders or crosslinking strategies were used. The internal microenvironment of this 3D construct was modified from solid to liquefied state by chelation. MTS and live{\textendash}dead assays showed enhanced L929 cell viability with liquefied 3D constructs, compared to non-liquefied ones. The proposed technique opens new prospects to create complex 3D polyelectrolyte based constructs for tissue engineering applications.</p>},
	keywords = {BEADS, cell microenvironments, ENCAPSULATION TECHNOLOGIES, Hydrogels, Multilayer capsules, SHAPE, TISSUE CONSTRUCTS},
	issn = {2046-2069},
	doi = {10.1039/C4RA11674G},
	url = {http://pubs.rsc.org/en/Content/ArticleLanding/2015/RA/C4RA11674G$\#$!divAbstract},
	author = {Praveen, S. and Correia, C. and Costa, R. R. and Mano, J. F.}
}
