@article {18287,
	title = {Assembly of cell-laden hydrogel fiber into non-liquefied and liquefied 3D spiral constructs by perfusion-based layer-by-layer technique},
	journal = {Biofabrication},
	volume = {7},
	year = {2015},
	month = {2015-01-06 00:00:00},
	pages = {011001-011007},
	publisher = {IOPscience},
	abstract = {<p style="text-align: justify;">In this work, three-dimensional (3D) self-sustaining, spiral-shaped constructs were produced\&nbsp;through a combination of ionotropic gelation, to form cell-encapsulated alginate fibers, and a perfusion-based\&nbsp;layer-by-layer (LbL) technique. Single fibers were assembled over cylindrical molds by\&nbsp;reeling to form spiral shapes, both having different geometries and sizes. An uninterrupted nanometric\&nbsp;multilayer coating produced by a perfusion-based LbL technique, using alginate and chitosan,\&nbsp;generated stable 3D spiral-shaped macrostructures by gripping and affixing the threads together without\&nbsp;using any crosslinking/binding agent. The chelation process altered the internal microenvironment\&nbsp;of the 3D construct from the solid to the liquefied state while preserving the external geometry.\&nbsp;L929 cell viability by MTS and dsDNA quantification favor liquefied 3D constructs more than nonliquefied\&nbsp;ones. The proposed technique setup helps us to generate complex polyelectrolyte-based 3D\&nbsp;constructs for tissue engineering applications and organ printing.</p>},
	keywords = {cell-encapsulation, chelation, hydrogel fiber, layer-by-layer, liquefied core, switching cell microenviroment, Tissue engineering},
	doi = {10.1088/1758-5090/7/1/011001},
	url = {http://iopscience.iop.org/1758-5090/7/1/011001/article},
	author = {Praveen, S. and Oliveira, S. M. and Borges, J. and Mano, J. F.}
}
