@inproceedings {18285, title = {Xenogeneic M2-polarization of Macrophages by Undifferentiated and Differentiated Adipose Tissue-derived Stem Cells}, journal = {Tissue Engineering Part A}, volume = {20}, year = {2014}, month = {2014-12-13 00:00:00}, pages = {S113-S114}, publisher = {Mary Ann Liebert, Inc.}, address = {Washington, DC}, abstract = {

Mesenchymal stem cells (MSCs) are considered to be {\textquoteleft}{\textquoteleft}immunologically\ privileged.{\textquoteright}{\textquoteright} In a previous work when human adipose\ tissue-derived stem cells (hASCs) subcutaneously implanted\ in mice we did not identify an adverse host response1. Recently, it\ was shown that tissue regeneration could benefit from the polarization
of M2 macrophages subpopulations 2. In this study we\ hypothesised that undifferentiated hASCs and derived osteoblasts\ and chondrocytes are able to switch murine bone marrow-derived\ macrophages (mBMM{\O}s) into M2 phenotype, aiding tissue regeneration.
Murine BMM{\O}s were plated in direct contact with undifferentiated\ and osteo or chondro-differentiated hASCs for 4 h, 10 h,\ 24 h and 72 h. The cytokine profile was analysed by qRT-PCR\ and the surface markers were detected by flow cytometry. The\ direct interaction of both cell types was observed by time lapse\ microscopy.
The results showed that mBMM{\O}s polarized after contacting tissue\ culture polystyrene. This M2 phenotype was maintained along the experiment\ in direct contact with both undifferentiated and osteo or\ chondro-differentiated hASCs. This was confirmed by the expression of\ IL-1, IL-10, IL-4, TNF-a and IFN-g (genetic profile) and surface\ markers (CD206 + + , CD336 + + , MHC II + and CD86 + + ) detection.\ These data suggest the potential of hASCs in contemporary xenogenic\ tissue engineering and regenerative medicine strategies, as\ well as host immune system modulation in autoimmune diseases.\ 

}, keywords = {Adipose tissue-derived stem cells, Immunomodulation}, doi = {10.1089/ten.tea.2014.5000.abstracts}, author = {Santos, T. C. and Rodrigues, D. B. and Cerqueira, M. T. and Pirraco, R. P. and Marques, A. P. and Reis, R. L.} }