@inproceedings {18205,
	title = {Chitosan - Alginate multilayered films with gradients of physical cues},
	journal = {Journal of Tissue Engineering and Regenerative Medicine},
	volume = {8},
	year = {2014},
	month = {2014-06-10 00:00:00},
	pages = {509},
	address = {Genova (Italy)},
	abstract = {<p>In general cell types are sensitive to mechanical stimulus of the surrounding environment which affects several cell properties. Thus, the development of biocompatible coatings with tuned mechanical properties are a current challenge, since gradients of physical signals are often applied in cellular processes to reconstruct the complex anisotropic environment presented by extracellular matrix (ECM) (1,2). Layer-by-layer (LbL) appears as a powerful tool since it allows the development of biomimetic multilayer films with well{\textendash}controlled structures at both the nano and micro-scales (2). This study aimed to develop chitosan (CHIT) and alginate (ALG) polyelectrolyte multilayers with gradients of physical cues generated by changing the reaction time of<br>genipin crosslinking as a function of controlled distance.</p>},
	keywords = {gradients, LbL multilayer assemblies},
	issn = {1932-6254},
	url = {http://onlinelibrary.wiley.com/doi/10.1002/term.1932/pdf},
	author = {Silva, J. M. and Oliveira, N. M. and Reis, R. L. and Mano, J. F.}
}
