@article {18173,
	title = {New biomaterial based on cotton with incorporated Biomolecules},
	journal = {Journal of Applied Polymer Science},
	volume = {131},
	year = {2014},
	month = {2014-11-05 00:00:00},
	abstract = {<p style="text-align: justify;">The aim of this study was to investigate a method of embedding l-cysteine (l-cys), an antimicrobial agent, between layers of chitosan (CH) and sodium alginate (ALG) onto cotton samples obtained via a layer-by-layer electrostatic deposition technique via several embedding methods. The results show that the best way to incorporate l-cys into the layers was the one that used the property of gelling ALG. To monitor the l-cys embedding into the CH/ALG multilayer film, different methods were used: energy-dispersive X-ray spectrometry analysis to assess the presence of sulfur on the sample, Ellman{\textquoteright}s reagent method to analyze l-cys release from the sample, and attenuated total reflectance (ATR) Fourier transform infrared spectroscopy (FTIR) to compare the ATR{\textendash}FTIR spectra of the pure l-cys and l-cys embedded in the CH/ALG multilayer film to study the interaction between the l-cys and the CH/ALG multilayer films. Functionalized CH/ALG cotton samples were also investigated for their antibacterial properties toward Staphylococcus aureus andKlebsiella pneumonia with the Japanese Industrial Standard method JIS L 1902:2002, and the results show an enhancement of the antibacterial effect due to the presence of l-cys.</p>},
	keywords = {Biomaterials, BIOMEDICAL APPLICATIONS, functionalization of polymers, self-assembly, textiles},
	doi = {10.1002/app.40519},
	author = {Gomes, A. P. and Mano, J. F. and Queiroz, J. A. and Gouveia, I. C.}
}
