@article {18146, title = {Differential regulation of osteogenic differentiation of stem cells on surface roughness gradients}, journal = {Biomaterials}, volume = {35}, year = {2014}, month = {2014-08-05 00:00:00}, pages = {9023-9032}, publisher = {Elsevier}, abstract = {

Tissue engineering using scaffold-cell constructs holds the potential to develop functional strategies to

regenerate bone. The interface of orthopedic implants with the host tissues is of great importance for its

later performance. Thus, the optimization of the implant surface in a way that could stimulate osteogenic

differentiation of mesenchymal stem cells (MSCs) is of significant therapeutic interest. The effect of

surface roughness of polycaprolactone (PCL) on the osteogenic differentiation of human bone-marrow

MSCs was investigated. We prepared surface roughness gradients of average roughness (Ra) varying

from the sub-micron to the micrometer range (~0.5e4.7 mm), and mean distance between peaks (RSm)

gradually varying from ~214 mm to 33 mm. We analyzed the degree of cytoskeleton spreading, expression

of alkaline phosphatase, collagen type 1 and mineralization. The response of cells to roughness divided

the gradient into three groups of elicited stem cell behavior: 1) faster osteogenic commitment and

strongest osteogenic expression; 2) slower osteogenic commitment but strong osteogenic expression,

and 3) similar or inferior osteogenic potential in comparison to the control material. The stem-cell

modulation by specific PCL roughness surfaces highlights the potential for creating effective solutions

for orthopedic applications featuring a clinically relevant biodegradable material.

}, keywords = {Mesenchymal stem cell, osteogenesis, polycaprolactone, Surface roughness}, doi = {10.1016/j.biomaterials.2014.07.015}, url = {http://www.sciencedirect.com/science/article/pii/S0142961214008096}, author = {Faia-Torres, A. B. and Guimond-Lischer, S. and Rottmar, M. and Charnley, M. and Goren, T. and Maniura-Weber, K. and Spencer, N. D. and Reis, R. L. and Textor, M. and Neves, N. M.} }