@article {18115,
	title = {Development of Anatomical Silk/Methacrylated Gellan Gum Scaffolds for Controlling Segmental Vascularization in Meniscus Tissue Regeneration},
	journal = {TERMIS-EU 2013},
	year = {2013},
	month = {2013-06-17 00:00:00},
	address = {Istanbul, Turkey},
	abstract = {<p>Meniscus tissue engineering can benefit from the use of highly developed scaffolds that are able to mimic the anatomy of this complex tissue, <em>i.e.</em> enable controlling the segmental vascularization during tissue regeneration. In this work, novel scaffolds were produced combining a porous silk polymeric matrix at 12 wt\% (silk-12) and the methacrylated gellan gum hydrogel (iGG-MA), which was previously shown to be non-angiogenic. A chorioallantoic membrane (CAM) assay was used to investigate the ability of the silk-12/iGG-MA scaffolds to control cell ingrowth, segmental vascularization, and innervations. Different sterile silk-based scaffolds were tested on the CAM, which were composed by silk-12 alone or combined with iGG-MA hydrogel and/or human meniscus cells. The angiogenic response was evaluated after 4 days of implantation by analysing the number of blood vessels converging toward the scaffolds, whereas possible inflammation and endothelial cells ingrowths{\textquoteright} was investigated by histological (haematoxylin and eosin - H\&amp;E - staining) and immunohistochemical methods (SNA-lectin staining). Results have shown that silk-12 are highly susceptible to cells ingrowths{\textquoteright} and blood vessel formation. However, when the hydrogel was combined with the silk-12, an inhibitory effect was observed as demonstrated by the low number of convergent blood vessels and less number of endothelial cells invading the silk-12/iGG-MA scaffolds. The iGG-MA hydrogel acted as a physical barrier for endothelial cells ingrowths{\textquoteright} and vascular invasion. This data showed that silk-12/iGG-MA scaffolds are not toxic, enabled controlling vascularisation and thus can be potentially applied in meniscus regeneration.</p>},
	keywords = {meniscus, Tissue engineering, vascularization},
	author = {Silva-Correia, J. and Pereira, H. and Yan, L. P. and Miranda-Gon{\c c}alves, V. and Espregueira-Mendes, J. D. and Oliveira, A. L. and Reis, R. M. and Oliveira, J. M. and Reis, R. L.}
}
