@article {18033,
	title = {Bone marrow stromal cells on a three-dimensional bioactive fiber mesh undergo osteogenic differentiation in the absence of osteogenic media supplements: The effect of silanol groups},
	journal = {Acta Biomaterialia},
	volume = {10},
	year = {2014},
	month = {2014-06-04 00:00:00},
	pages = {4175-4185},
	publisher = {Elsevier },
	abstract = {<p><span>Osteogenic differentiation is a tightly regulated process dependent on the stimuli provided by the micro-environment. Silicon-substituted materials are known to have an influence on the osteogenic phenotype of undifferentiated and bone-derived cells. This study aims to investigate the bioactivity profile as well as the mechanical properties of a blend of starch and poly-caprolactone (SPCL) polymeric fiber mesh scaffolds functionalized with silanol (Si{\textendash}OH) groups as key features for bone tissue engineering strategies. The scaffolds were made from SPCL by a wet spinning technique. A calcium silicate solution was used as a non-solvent to develop an in situ functionalization with Si{\textendash}OH groups in a single-step approach. We also explored the relevance of silicon incorporated in SPCL{\textendash}Si scaffolds to the in vitro osteogenic process of goat bone marrow stromal cells (gBMSCs) with and without osteogenic supplements in the culture medium. We hypothesized that SPCL{\textendash}Si scaffolds could act as physical and chemical millieus to induce per se the osteogenic differentiation of gBMSCs. Results show that osteogenic differentiation of gBMSCs and the production of a mineralized extracellular matrix on bioactive SPCL{\textendash}Si scaffolds occur for up to 2\&nbsp;weeks, even in the absence of osteogenic supplements in the culture medium. The omission of media supplements to induce osteogenic differentiation is a promising feature towards simplified and cost-effective cell culturing procedures of a potential bioengineered product, and concomitant translation into the clinical field. Thus, the present work demonstrates that SPCL{\textendash}Si scaffolds and their intrinsic properties sustain gBMSC osteogenic features in vitro, even in the absence of osteogenic supplements to the culture medium, and show great potential for bone regeneration strategies.</span></p>},
	keywords = {apatite, Goat bone marrow mesenchymal cells, osteogenic differentiation, silanol groups, wet-spinning},
	doi = {10.1016/j.actbio.2014.05.026},
	url = {http://www.sciencedirect.com/science/article/pii/S1742706114002384$\#$},
	author = {Rodrigues, M. T. and Leonor, I. B. and Gr{\"o}ena, N. and Viegas, C. A. and Dias, I. R. and Caridade, S. G. and Mano, J. F. and Gomes, M. E. and Reis, R. L.}
}
