@article {18020,
	title = {A Tissue Engineering Approach for Periodontal Regeneration Based on a Biodegradable Double-Layer Scaffold and Adipose-Derived Stem Cells},
	journal = {Tissue Engineering: Part A},
	volume = {20},
	year = {2014},
	month = {2014-09-11 00:00:00},
	pages = {17-18},
	publisher = {Mary Ann Liebert, Inc},
	abstract = {<p class="p1">Human and canine periodontium are often affected by an inflammatory pathology called periodontitis, which is\&nbsp;associated with severe damages across tissues, namely, in the periodontal ligament, cementum, and alveolar\&nbsp;bone. However, the therapies used in the routine dental practice, often consisting in a combination of different\&nbsp;techniques, do not allow to fully restore the functionality of the periodontium. Tissue Engineering (TE) appears\&nbsp;as a valuable alternative approach to regenerate periodontal defects, but for this purpose, it is essential to\&nbsp;develop supportive biomaterial and stem cell sourcing/culturing methodologies that address the complexity of\&nbsp;the various tissues affected by this condition. The main aim of this work was to study the in vitro functionality\&nbsp;of a newly developed double-layer scaffold for periodontal TE. The scaffold design was based on a combination\&nbsp;of a three-dimensional (3D) fiber mesh functionalized with silanol groups and a membrane, both made of a\&nbsp;blend of starch and poly-e-(caprolactone). Adipose-derived stem cells (canine adipose stem cells [cASCs]) were\&nbsp;seeded and cultured onto such scaffolds, and the obtained constructs were evaluated in terms of cellular\&nbsp;morphology, metabolic activity, and proliferation. The osteogenic potential of the fiber mesh layer functionalized\&nbsp;with silanol groups was further assessed concerning the osteogenic differentiation of the seeded and\&nbsp;cultured ASCs. The obtained results showed that the proposed double-layer scaffold supports the proliferation\&nbsp;and selectively promotes the osteogenic differentiation of cASCs seeded onto the functionalized mesh. These\&nbsp;findings suggest that the 3D structure and asymmetric composition of the scaffold in combination with stem\&nbsp;cells may provide the basis for developing alternative therapies to treat periodontal defects more efficiently.</p>},
	keywords = {Double layer scaffold, Periodontal regeneration, tissue engineering.},
	doi = {10.1089/ten.tea.2013.0360},
	url = {http://online.liebertpub.com/doi/abs/10.1089/ten.TEA.2013.0360?url_ver=Z39.88-2003\&rfr_id=ori:rid:crossref.org\&rfr_dat=cr_pub\%3dpubmed},
	author = {Requicha, J. F. and Viegas, C. A. and Mu{\~n}oz, F. and Azevedo, J. M. and Leonor, I. B. and Reis, R. L. and Gomes, M. E.}
}
