@article {18006,
	title = {Free and copolymerized gamma-cyclodextrins regulate performance of dexamethasone-loaded dextran microspheres for bone regeneration},
	journal = {Journal of Materials Chemistry B},
	volume = {2},
	year = {2014},
	month = {2014-05-24 00:00:00},
	pages = {4943-4956},
	publisher = {The Royal Society of Chemistry},
	abstract = {<p><span>Polymeric particles acting as source of biological cues to promote tissue regeneration are currently an interesting topic in bone tissue engineering research. In this study, microspheres of dextran-methacrylated (dextran-MA) and γ-cyclodextrins (γ-CD) for the delivery of osteogenic agents were prepared by means of photopolymerization on biomimetic superhydrophobic surfaces. The effects of the incorporation of the γ-CD units as free entities or as structural monomers (acrylamidomethyl-γ-cyclodextrin, γ-CD-NMA) on dexamethasone loading and release performance were evaluated in detail in order to achieve osteogenic differentiation of human stem cells. The copolymerization of dextran-MA with γ-CD-NMA improved the loading capacity of the particles and also provided a sustained release of dexamethasone for several days. The biological studies revealed that such microspheres were cytocompatible and capable to induce the differentiation of human adipose-derived stem cells (hASCs) to osteoblasts, as determined from an increase of alkaline phosphatase (ALP) activity between days 3 and 7. Such results were also confirmed using ALP staining. Therefore, immobilization of γ-CDs onto dextran-MA network may be particularly useful for the development of cytocompatible implantable spherical biomaterials for bone tissue engineering purposes.</span></p>},
	keywords = {cyclodextrins, dextran, microspheres, superhydrophobic surfaces},
	doi = {10.1039/C3TB21665A},
	url = {http://pubs.rsc.org/en/content/articlelanding/2014/tb/c3tb21665a$\#$!divAbstract},
	author = {Lima, A. C. and Puga, A. M. and Mano, J. F. and Concheiro, A. and Alvarez-Lorenzo, C.}
}
