@article {17970, title = {Volatile composition of Catharanthus roseus (L.) G. Don using solid-phase microextraction and gas chromatography/mass spectrometry}, journal = {Journal of Pharmaceutical \& Biomedical Analysis}, volume = {49}, year = {2009}, month = {2009-04-05 00:00:00}, pages = {674-685}, abstract = {

A total of 88 volatile and semi-volatile components were formally or tentatively identified in flowers, leaves and stems of\ Catharanthus roseus\ (L.) G. Don (cv. Little Bright Eye), by headspace solid-phase microextraction (HS-SPME) and by dichloromethane extraction, combined with gas chromatography{\textendash}mass spectrometry (GC{\textendash}MS). These include some diterpenic compounds (manool and manoyl oxides), a sesquiterpen (α-bisabolol), and some pyridine, pyrazine, indol and carotenoid derivatives. Applying multivariate analysis (principal component analysis and agglomerative hierarchic cluster analysis) to the HS-SPME{\textendash}GC{\textendash}MS data, it was possible to characterize each part of the vegetal material using a relative small number of compounds. Hence, flowers were richer in terpenic molecules (including limonene), α-bisabolol, methyljasmonate,\ cis-jasmone, 2-phenylethanol, phenylacetaldehyde,\ trans-2-octenal, benzylic alcohol and 2-isobutyl-3-methoxypyrazine. Leaves can be characterized by the methyl and propyl esters of fatty acids, mono- and disaturated,\ trans-phytol, carotenoid derivative compounds, hydrofarnesylacetone, methylanthranilate, manool and epi-manool oxide, while stems have high levels of volatile aldehydes, such as hexanal, octanal,\ cis-2-nonenal,\ cis-2-decenal,\ cis,\ trans-2,6-nonadienal,\ trans,\ trans-2,4-decadienal andcis,\ trans-2,4-decadienal. Dichloromethane extraction allowed also the identification of some alkaloid-like compounds that were not detected by HS-SPME.

}, keywords = {GC{\textendash}MS, HS-SPME, Volatiles and semi-volatiles}, author = {Guedes de Pinho, P. and Gon{\c c}alves, R. F. and Valent{\~a}o, P. and Pereira, D. M. and Seabra, R. M. and Andrade, P. B. and Sottomayor, M.} }