@article {17949, title = {In Vivo High-Content Evaluation of Three-Dimensional Scaffolds Biocompatibility}, journal = {Tissue Engineering Part C - Methods}, year = {2014}, month = {2014-03-31 00:00:00}, publisher = {Mary Ann Liebert, Inc}, abstract = {

While developing tissue engineering strategies, inflammatory response caused by biomaterials is an unavoidable aspect to be taken into consideration, as it may be an early limiting step of tissue regeneration approaches. We demonstrate the application of flat and flexible films exhibiting patterned high-contrast wettability regions as implantable platforms for the high-content\ in vivo\ study of inflammatory response caused by biomaterials. Screening biomaterials by using high-throughput platforms is a powerful method to detect hit spots with promising properties and to exclude uninteresting conditions for targeted applications. High-content analysis of biomaterials has been mostly restricted to\ in vitro\ tests where crucial information is lost, as\ in vivo\ environment is highly complex. Conventional biomaterials implantation requires the use of high numbers of animals, leading to ethical questions and costly experimentation. Inflammatory response of biomaterials has also been highly neglected in high-throughput studies. We designed an array of 36 combinations of biomaterials based on an initial library of four polysaccharides. Biomaterials were dispensed onto biomimetic superhydrophobic platforms with wettable regions and processed as freeze-dried three-dimensional scaffolds with a high control of the array configuration. These chips were afterward implanted subcutaneously in Wistar rats. Lymphocyte recruitment and activated macrophages were studied on-chip, by performing immunocytochemistry in the miniaturized biomaterials after 24 h and 7 days of implantation. Histological cuts of the surrounding tissue of the implants were also analyzed. Localized and independent inflammatory responses were detected. The integration of these data with control data proved that these chips are robust platforms for the rapid screening of early-stage\ in vivo\ biomaterials{\textquoteright} response.

}, keywords = {High throughput analysis, superhydrophobic}, doi = {10.1089/ten.tec.2013.0738}, url = {http://online.liebertpub.com/doi/abs/10.1089/ten.TEC.2013.0738?url_ver=Z39.88-2003\&rfr_id=ori:rid:crossref.org\&rfr_dat=cr_pub\%3dpubmed}, author = {Oliveira, M. B. and Ribeiro, M. P. and Miguel, S. P. and Neto, A. I. and Coutinho, P. and Correia, I. J. and Mano, J. F.} }