@article {17945, title = {Palmitic acid and ergosta-7,22-dien-3-ol contribute to the ER-stress-mediated apoptotic effect and cell cycle arrest of an extract from Marthasterias glacialis L. in neuroblastoma cells.}, journal = {Marine Drugs}, volume = {12}, year = {2014}, month = {2014-01-01 00:00:00}, pages = {54-68}, abstract = {

We describe the effect of a chemically characterized lipophilic extract obtained from Marthasterias glacialis L. against human breast cancer (MCF-7) and human neuroblastoma (SH-SY5Y) cell lines. Evaluation of DNA synthesis revealed that both cell lines were markedly affected in a concentration-dependent way, SH-SY5Y cell line being more susceptible. Cell cycle arrest was observed, an effect induced by the sterol ergosta-7,22-dien-3-ol present in the extract. Morphological evaluation of treated cells showed the advent of lipid droplets and chromatin condensation compatible with apoptosis, which was confirmed by the evaluation of caspase-3 and -9 activities. Palmitic acid was the main compound responsible for this apoptotic effect by a ceramide-independent mechanism that involved activation of the CHOP-mediated ER-stress pathway.

}, keywords = {Apoptosis, CHOP, ER-stress, Marthasterias glacialis L., Palmitic acid}, author = {Pereira, D. M. and Correia-da-Silva, G. and Valent{\~a}o, P. and Teixeira, N. and Andrade, P. B.} }