@article {17914,
	title = {Cellular Uptake of Multilayered Capsules Produced with Biomimetic and Genetically Engineered Macromolecules},
	journal = {Acta Biomaterialia},
	volume = {10},
	year = {2014},
	month = {2014-02-20 00:00:00},
	pages = {2653-2662},
	publisher = {Elsevier},
	abstract = {<p><span>Multilayered microcapsules of chitosan and biomimetic elastin-like recombinamers (ELRs) were prepared envisaging the intracellular delivery of active agents. Two ELRs containing either a bioactive RGD sequence or a scrambled non-functional RDG were used to construct two types of functionalized polymeric microcapsules, both of spherical shape \~{}4\&nbsp;μm in diameter. Cell viability studies with human mesenchymal stem cells (hMSCs) were performed using microcapsule/cell ratios between 5:1 and 100:1. After 3 and 72\&nbsp;h of co-incubation, no signs of cytotoxicity were found, but cells incubated with RGD-functionalized microcapsules exhibited higher viability values than RDG cells. The internalization efficacy and bioavailability of encapsulated DQ-ovalbumin were assessed by monitoring the fluorescence changes in the cargo. The data show that surface functionalization did not significantly influence internalization by hMSCs, but the bioavailability of DQ-ovalbumin degraded faster when encapsulated within RGD-functionalized microcapsules. The microcapsules developed show promise for intracellular drug delivery with increased drug efficacy.</span></p>},
	keywords = {Biomaterials, Cellular uptake, layer-by-layer, Multilayer capsules},
	doi = {10.1016/j.actbio.2014.02.020},
	url = {http://www.sciencedirect.com/science/article/pii/S1742706114000725$\#$},
	author = {Costa, R. R. and Girotti, A. and Santos, M. and Arias, F. J. and Mano, J. F. and Rodr{\'\i}guez-Cabello, J. C.}
}
