@article {17904,
	title = {Controlled Release Strategies for Bone, Cartilage, and Osteochondral Engineering{\textemdash}Part II: Challenges on the Evolution from Single to Multiple Bioactive Factor Delivery},
	journal = {Tissue Engineering: Part B},
	volume = {19},
	year = {2013},
	month = {2013-06-20 00:00:00},
	pages = {327-352},
	publisher = {Mary Ann Liebert, Inc.},
	abstract = {<p class="p1">\&nbsp;The development of controlled release systems for the regeneration of bone, cartilage, and osteochondral interface\&nbsp;is one of the hot topics in the field of tissue engineering and regenerative medicine. However, the majority\&nbsp;of the developed systems consider only the release of a single growth factor, which is a limiting step for the\&nbsp;success of the therapy. More recent studies have been focused on the design and tailoring of appropriate\&nbsp;combinations of bioactive factors to match the desired goals regarding tissue regeneration. In fact, considering\&nbsp;the complexity of extracellular matrix and the diversity of growth factors and cytokines involved in each\&nbsp;biological response, it is expected that an appropriate combination of bioactive factors could lead to more\&nbsp;successful outcomes in tissue regeneration. In this review, the evolution on the development of dual and\&nbsp;multiple bioactive factor release systems for bone, cartilage, and osteochondral interface is overviewed, specifically\&nbsp;the relevance of parameters such as dosage and spatiotemporal distribution of bioactive factors. A\&nbsp;comprehensive collection of studies focused on the delivery of bioactive factors is also presented while highlighting\&nbsp;the increasing impact of platelet-rich plasma as an autologous source of multiple growth factors.</p>},
	keywords = {controlled release, tisssue engineering},
	doi = {10.1089/ten.TEB.2012.0727},
	url = {http://online.liebertpub.com/doi/abs/10.1089/ten.TEB.2012.0727?url_ver=Z39.88-2003\&rfr_id=ori:rid:crossref.org\&rfr_dat=cr_pub\%3dpubmed},
	author = {Santo, V. E. and Gomes, M. E. and Mano, J. F. and Reis, R. L.}
}
