@article {17872,
	title = {Molecular characterization of extended-spectrum-beta-lactamase-producing Escherichia coli isolates from red foxes in Portugal},
	journal = {Archives of Microbiology},
	year = {2012},
	month = {2012-11-22 00:00:00},
	abstract = {<p>The presence of broad-spectrum-cephalosporin-\&nbsp;resistant Escherichia coli\&nbsp; isolates and the implicated\&nbsp;mechanisms of resistance and virulence factor genes\&nbsp;were investigated in red fox (Vulpes vulpes ) in Portugal.\&nbsp;Cefotaxime-resistant E. coli\&nbsp; isolates were isolated from\&nbsp;two of 52 fecal samples (4 \%), being both ESBL producers.\&nbsp;The <strong>b</strong> -lactamase genes found in the two isolates were\&nbsp;blaSHV-12+blaTEM-1b . The tet (A) and sul 2 genes were\&nbsp;also detected in these isolates, together with the non-classical\&nbsp;class 1 integron (intI1 -dfr A12-orf F-aad A2-cml A1-\&nbsp;aad A1-qacH -IS440 -sul 3) with the PcH1 promoter. The\&nbsp;two isolates belonged to the phylogroup A. Amino acid\&nbsp;changes in GyrA (S83L + D87G) and ParC (S80I) proteins\&nbsp;were identified in our study. Concerning MLST typing,\&nbsp;both isolates were assigned to ST1086, never found before\&nbsp;in wild animals, and they presented closely related PFGE\&nbsp;patterns. This study reveals the presence of ESBL-producing\&nbsp;E. coli\&nbsp; isolates, in a wild ecosystem, which could be\&nbsp;disseminated through the environment to other niches.\&nbsp;</p>},
	keywords = {E. coli, ESBL, Phylogenetic groups, Virulence factors, Vulpes vulpes},
	doi = {10.1007/s00203-012-0853-7},
	author = {Radhouani, H. and Igrejas, G. and Gon{\c c}alves, A. and Estepa, R. and Sargo, R. and Torres, C. and Poeta, P.}
}
