@article {17867, title = {After genomics, what proteomics tools could help us understand the antimicrobial resistance of Escherichia coli? }, journal = {Journal of Proteomics}, year = {2012}, month = {2012-12-31 00:00:00}, abstract = {

Proteomic approaches have been considerably improved during the past decade and have

been used to investigate the differences in protein expression profiles of cells grown

under a broad spectrum of growth conditions and with different stress factors including

antibiotics.

In Europe, the most significant disease threat remains the presence of microorganisms that

have become resistant to antimicrobials and so it is important that different scientific tools

are combined to achieve the largest amount of knowledge in this area of expertise. The

emergence and spread of the antibiotic-resistant Gram-negative pathogens, such as

Escherichia coli, can lead to serious problem public health in humans. E. coli, a very well

described prokaryote, has served as a model organism for several biological and biotechnological

studies increasingly so since the completion of the E. coli genome-sequencing

project. The purpose of this review is to present an overview of the different proteomic

approaches to antimicrobial-resistant E. coli that will be helpful to obtain a better knowledge

of the antibiotic-resistant mechanism(s). This can also aid to understand the molecular

determinants involved with pathogenesis, which is essential for the development of effective

strategies to combat infection and to reveal new therapeutic targets. This article is part

of a Special Issue entitled: Proteomics: The clinical link.

}, keywords = {Antimicrobial resistance, Clinical isolates, E. coli, Genomics, Proteomics}, author = {Radhouani, H. and Pinto, L. and Poeta, P. and Igrejas, G.} }