@article {17791, title = {The interactions between exogenous FGF-2 and sulfonic groups: Impact on the morphology of human adipose-derived stem cells}, booktitle = {TERMIS-EU}, year = {2013}, month = {2013-06-17 00:00:00}, address = {Istanbul, Turkey}, abstract = {

FGF-2 is often an additive to stem cells culture media aimed at preserving the differentiation potential of stem cells through higher passages. Its bioactivity is triggered by binding two classes of receptors: high-affinity fibroblast growth factor receptors and low-affinity receptors {\textendash} heparin and heparan sulfate proteoglycans (HSPG). The sulfation degree and positioning in HSPG play a crucial role in these processes. In this work, we have prepared synthetic mimics of HSPG with different content of {\textendash}SO3H and {\textendash}OH groups. Mixed self-assembled monolayers were prepared by co-adsorption of HS(CH2)11OH and HS(CH2)11SO3H at different ratios (0.00, 0.25, 0.75 and 1.00). The interaction between these surfaces and FGF- 2 was studied in situ by QCM-D: in the presence of {\textendash}OH groups the adsorbed mass increases with the rise of the {\textendash}SO3H surface fraction. This mass increase is not a function of FGF-2 concentration but the kinetics of the process is concentration-dependent. Surfaces containing only {\textendash}SO3H groups demonstrated unexpected behaviour: a lower amount of FGF- 2 was detected for these substrates as compared to the ones with -SO3H surface fraction of 0.75. The bioactivity of FGF-2 was tested by its interactions with anti-FGF-2 and decreases in the following order: -SO3H(0.75) \> -SO3H(1.00) \>\> -SO3H(0.25) \> -SO3H(0.00). Finally, we investigated the impact of these interactions on adipose-derived stem cells (ASCs) morphology. We found that in addition to spindle-like cellular morphology, the exogenous FGF-2 induces significantly more and longer filopodia in ASCs cultured on {\textendash}SO3H rich surfaces.

}, keywords = {adipose derived stem cells, FGF-2, self-assembled monolayers}, author = {Amorim, S. and Pires, R. A. and Soares da Costa, D. and Reis, R. L. and Pashkuleva, I.} }