@article {17674,
	title = {Undifferentiated human adipose-derived stromal/stem cells loaded onto wet-spun starch-polycaprolactone scaffolds enhance bone regeneration: Nude mice calvarial defect in vivo study},
	journal = {Journal of Biomedical Materials and Research - Part A},
	year = {2013},
	month = {2013-10-12 00:00:00},
	pages = {[Epub ahead of print]},
	publisher = {Wiley Periodicals, Inc},
	abstract = {<p class="p1" style="text-align: justify;">The repair of large bony defects remains challenging\&nbsp;in the clinical setting. Human adipose-derived stromal/stem cells (hASCs) have been reported to differentiate along\&nbsp;different cell lineages, including the osteogenic. The objective\&nbsp;of the present study was to assess the bone regeneration\&nbsp;potential of undifferentiated hASCs loaded in starchpolycaprolactone\&nbsp;(SPCL) scaffolds, in a critical-sized nude\&nbsp;mice calvarial defect. Human ASCs were isolated from lipoaspirate\&nbsp;of five female donors, cryopreserved, and pooled\&nbsp;together. Critical-sized (4 mm) calvarial defects were created\&nbsp;in the parietal bone of adult male nude mice. Defects were\&nbsp;either left empty, treated with an SPCL scaffold alone, or\&nbsp;SPCL scaffold with human ASCs. Histological analysis and\&nbsp;Micro-CT imaging of the retrieved implants were performed.\&nbsp;Improved new bone deposition and osseointegration was\&nbsp;observed in SPCL loaded with hASC engrafted calvarial\&nbsp;defects as compared to control groups that showed little\&nbsp;healing. Nondifferentiated human ASCs enhance ossification\&nbsp;of nonhealing nude mice calvarial defects, and wet-spun\&nbsp;SPCL confirmed its suitability for bone tissue engineering.\&nbsp;This study supports the potential translation for ASC use\&nbsp;in the treatment of human skeletal defects.</p>},
	keywords = {adipose-derived stem cells, bone regeneration, calvarial defect, critical size defect, SPCL},
	doi = {DOI: 10.1002/jbm.a.34983},
	url = {http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.34983/abstract},
	author = {Carvalho, P. P. and Leonor, I. B. and Smith, B. J. and Dias, I. R. and Reis, R. L. and Gimble, J. M. and Gomes, M. E.}
}
