@article {17649,
	title = {Porous hydrogels from shark skin collagen crosslinked under dense carbon dioxide atmosphere},
	journal = {Macromolecular Bioscience},
	volume = {13},
	year = {2013},
	month = {2013-08-30 00:00:00},
	pages = {1621-1631},
	publisher = {Wiley},
	abstract = {<p>The possibility to fabricate marine collagen porous structures crosslinked with genipin under high pressure carbon dioxide is investigated. Collagen from shark skin is used to prepare pre-scaffolds by freeze-drying. The poor stability of the structures and low mechanical properties require crosslinking of the structures. Under dense CO<sub><span style="font-size: small;">2</span></sub> atmosphere, crosslinking of collagen pre-scaffolds is allowed for 16 h. Additionally, the hydrogels are foamed and the scaffolds obtained present a highly porous structure. In vitro cell culture tests performed with a chondrocyte-like cell line show good cell adherence and proliferation, which is a strong indication of the potential of these scaffolds to be used in tissue cartilage tissue engineering.</p>},
	keywords = {Collagen, Hydrogels, marine biotechnolgoy, supercritical fluids, Tissue engineering},
	url = {http://onlinelibrary.wiley.com/doi/10.1002/mabi.201300228/abstract},
	author = {Silva, S. P. and Moreira-Silva, J. and Silva, T. H. and Perez-Martin, R. I. and Sotelo, C. G. and Mano, J. F. and Duarte, A. R. C. and Reis, R. L.}
}
