@article {17604, title = {Secondary Structure of rhBMP-2 in a Protective Biopolymeric Carrier Material}, journal = {Biomacromolecules}, volume = {13}, year = {2012}, month = {2012-11-01 00:00:00}, pages = {3620-3626}, publisher = {AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA}, abstract = {

Efficient delivery,Of\ growth factors is one\ of\ the great challenges,\ of\ tissue engineering. Polyelectrolyte multi layer films (PEM) made\ ofbiopolymers have recently emerged as an interesting\ carrier\ for delivering recombinant human bone morphogenetic protein 2 (rhBMP-2 noted here BMP-2) to cells\ ina\ matrix bound manner: We recently showed that PEM made\ Of\ poly(1,:lysine) and hyaluronan (PLL/HA) Can retain high and tunable quantities\ ofBMP-2 and can deliver it to cells to induce their differentiation\ in\ osteoblasts. Here, we investigate quantitatively by Fourier, transform infrared spectroscopy (FTIR) the\ secondarystructureof\ BMP-2\ in\ solution as Well as trapped,in,abiopolymeric\ thin filth. We reveal that the major structural L elements\ of\ BMP-2\ insolution are intramolecular beta-sheets and unordered. structures as well as alpha-helices Furthermore, We studied the\ secondarystructureof\ rhBMP-1 trapped\ in\ :hydrated : films and\ in\ dry films since drying is an important step for future applications\ of\ these bioactive films onto orthopedic biomaterials. We demonstrate that the structural elements were preserved when BMP-2 was trapped\ in\ the\ biopolymeric-film\ in\ hydrated conditions and, to\ a\ lesser extent,\ indry state. Importantly, its bioactivity was maintained after drying\ of\ the film. Our results appear highly promising for future applications\ of\ these films as coatings\ of\ biomedical materials, to deliver bioactive proteins while preserving their bioactivity upon storage\ in\ dry state.

}, keywords = {ATR-FTIR, DIFFERENTIATION, GROWTH-FACTORS, INHIBITION, MICROENVIRONMENTS, ONE MORPHOGENETIC PROTEIN-2, POLYELECTROLYTE MULTILAYER FILMS, STABILITY, TISSUE; BMP-2}, issn = {1525-7797}, doi = {10.1021/bm3010808}, url = {http://apps.webofknowledge.com/full_record.do?product=UA\&search_mode=GeneralSearch\&qid=15\&SID=R26mYWbF6MIAgV5us3R\&page=1\&doc=1}, author = {Gilde, F. and Maniti, O. and Guillot, R. and Mano, J. F. and Logeart-Avramoglou, D. and Sailhan, F. and Picart, C} }