@article {17602, title = {Enzymatic degradation behavior and cytocompatibility of silk fibroin{\textendash}starch{\textendash}chitosan conjugate membranes}, journal = {MATERIALS SCIENCE \& ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS}, volume = {32}, year = {2012}, month = {2012-08-01 00:00:00}, pages = {1314-1322}, publisher = {ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS}, abstract = {

The objective\ of\ this study was to investigate the influence\ ofsilk\ fibroin and oxidized starch conjugation on the\ enzymaticdegradationbehaviorand the cytocompatability\ of\ chitosan based biomaterials. The tensile stress\ ofconjugatemembranes, which was at 50 Megapascal (MPa) for the lowest fibroin and starch composition (10 weight percent (wt.\%)), was decreased significantly with the increased content\ of\ fibroin and starch. The weight loss\ ofconjugates in alpha-amylase was more notable when the starch concentration was the highest at 30 wt.\%. The conjugates were resistant to the\ degradationby protease and lysozyme except for the conjugates with the lowest starch concentration. After 10 days\ of\ cell culture, the proliferation\ of\ osteoblast-like cells (SaOS-2) was stimulated significantly by higher fibroin compositions and the DNA synthesis on the\ conjugate\ with the highest fibroin (30 wt.\%) was about two times more compared to the native chitosan. The light microscopy and the image analysis results showed that the cell area and the lengths were decreased significantly with higher fibroin/chitosan ratio. The study proved that the conjugation\ of\ fibroin and starch with the chitosan based biomaterials by the use\ of\ non-toxic reductive alkylation crosslinking significantly improved the\ cytocompatibility\ and modulated the biodegradation, respectively.

}, keywords = {Biodegradation, Chitosan, Conjugation, Cytocompatibility, Silk Fibroin, Starch}, issn = {0928-4931}, doi = {10.1016/j.msec.2012.02.015}, url = {http://apps.webofknowledge.com/full_record.do?product=UA\&search_mode=GeneralSearch\&qid=13\&SID=R26mYWbF6MIAgV5us3R\&page=1\&doc=1}, author = {Baran, E. T. and Tuzlakoglu, K. and Mano, J. F. and Reis, R. L.} }