@article {17597, title = {Different hyaluronic acid morphology modulates primary articular chondrocyte behavior in hyaluronic acid-coated polycaprolactone scaffolds}, journal = {JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A}, volume = {101A}, year = {2013}, month = {2013-02-01 00:00:00}, pages = {518-527}, publisher = {WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA}, abstract = {

Scaffolds\ for cartilage tissue engineering should promote both adequate biomechanical environment and chondrogenic stimulation.\ Hyaluronicacid\ (HA) has been used\ in\ cartilage engineering for its chondrogenic and chondroprotective properties, nevertheless its mechanical properties are limited. Influence of HA microstructure\ inchondrocyte\ response has not been addressed yet.\ In\ this work,\ polycaprolactone\ (PCL)\ scaffolds\ were modified using HA following two coating strategies: coating\ in\ one step (PCL-HA1s) yields a gel-like phase within the scaffold, whereas a two-step reaction (PCL-HA2s) yields a thin HA layer coating internal surfaces of PCL structure. Chondrocytes were seeded\ in\ the\ scaffolds\ and cultured\ in\ dedifferentiating conditions up to 3 weeks and analyzed using a total DNA assay and sulfated glycosaminoglycan (sGAG) determination assay; cell\ morphology\ and extracellular matrix secretion were assessed by electron microscopy as well as immunofluorescent imaging (collagen I, collagen II, aggrecan, CD44). Cells proliferate\ in\ all samples and no cytotoxicity is observed. PCL-HA1s shows higher sGAG production per cell than PCL and PCL-HA2s at all times. Presence of\ hyaluronicacid\ promotes qualitative expression of CD44 surface markers and aggrecan (more visible\ in\ PCL-HA1s than PCL-HA2s), whereas\ in\ dedifferentiating conditions, expression of CD44 and aggrecan can hardly be detected\ in\ pure PCL\ scaffolds. Collagen type II seems more prominent\ in\ PCL-HA2s; although PCL-HA2s shows markers for COL II, aggrecan and CD44, quantitative ECM production is not improved with respect to PCL. It is thus likely that CD44 activation is not sufficient for explaining the better response\ in\ PCL-HA1s.

}, keywords = {cartilage, human chondrocyte, polycaprolactone, Tissue engineering}, issn = {1549-3296}, doi = {10.1002/jbm.a.34349}, url = {http://apps.webofknowledge.com/full_record.do?product=UA\&search_mode=GeneralSearch\&qid=4\&SID=R26mYWbF6MIAgV5us3R\&page=1\&doc=1}, author = {Lebourg, M. and Rochina, J. R. and Sousa, T. and Mano, J. F. and Ribelles, J. L. G.} }