@article {17554,
	title = {Interactions between exogenous FGF-2 and sulfonic groups: in situ characterization and impact on the morphology of human adipose-derived stem cells},
	journal = {Langmuir},
	volume = {29},
	year = {2013},
	month = {2013-05-31 00:00:00},
	pages = {7983-7992},
	publisher = {American Chemical Society},
	abstract = {<p>FGF-2 is often used as a supplement to stem cells culture medium aiming at preserving their self-renewal capacity and plasticity through the passages. However, little is known on the influence of the underlying substrate in these interactions. In this study, we have used mixed self-assembled monolayers with different ratios of {\textendash}SO3H and {\textendash}OH tail groups to investigate the influence of substrate properties (e.g. charge) on the FGF-2 adsorption and activity. QCM-D data demonstrated that in the presence of {\textendash}OH groups, the quantity of the adsorbed FGF-2 is proportional to the percentage of surface {\textendash}SO3H groups. The bioactivity of the adsorbed FGF-2 follows the same tendency as demonstrated by its interactions with anti-FGF-2. Surprisingly, the adlayer of FGF-2 formed on surface containing only SO3H-tailed SAMs was similar to the surface with 25\% of {\textendash}SO3H groups, demonstrating that FGF-2 adsorption is not solely driven by electrostatic interactions. We related these results with changes in the morphology of adipose-derived stem cells (ASCs) cultured on the same surfaces.</p>},
	keywords = {adipose-derived stem cells, FGF-2, quartz crystal microbalance, self-assembled monolayers, streaming potential},
	doi = {10.1021/la400871c},
	url = {http://pubs.acs.org/doi/abs/10.1021/la400871c},
	author = {Amorim, S. and Pires, R. A. and Soares da Costa, D. and Reis, R. L. and Pashkuleva, I.}
}
