@inproceedings {17497, title = {Sulfation degree of glycosaminoglycans triggers distinct cytoskeleton organisation in mesenchymal stem cells}, journal = { Journal of Tissue Engineering and Regenerative Medicine}, volume = {6}, year = {2012}, month = {2012-10-31 00:00:00}, pages = {21-21}, publisher = {Wiley \& Sons, Inc.}, address = {Guimar{\~a}es, Portugal}, abstract = {

Glycosaminoglycans (GAGs) comprise the closest cellular environment: they are building
elements of the ECM and can be also found on cells surface. Their biological activity depends
on several parameters among which the negative charge is of prime importance[1]. This
charge is generally associated with the presence of sulfate groups (-OSO3H). Sulfation is
a dynamic modification: it can occur at various positions within the glycan and different
sulfation patterns have been identified for the same organs and cells during their
development. However, the mechanisms of coding and transferring information by
these functionalities are not yet complete understood, mainly because of (i)the complex
physiological microenvironment in which GAGs interactions occur and (ii)the inability to access
homogeneous GAGs[2].
In this work, we propose model surfaces bearing GAGs with different sulfation degree as
platform to investigate the pathways by which mesenchymal stem cells (MSCs) sense and
respond to this peculiar functionality: the -OSO3H. We have selected two natural GAGs for this
study: hyaluronic acid (HA) because it is the only non-sulfated glycan and heparin (HEP) as it is
the GAG with the highest degree of sulfation. To obtain a larger range of sulfation degrees, we
have also prepared a synthetic analogue of HA with a sulfation degree of 1.4 (sHA). All these
GAGs were covalently bonded to aminothiols deposited on gold surfaces. MSCs, both from
bone marrow and adipose tissue, adhered well to all surfaces. Formation of focal adhesions
was observed after only 1h of culture for bone marrow derived MSCs regardless the used
substrate. The presence of {\textendash}OSO3H groups induced different morphology and cytoskeleton
organisation: formation of longer filopodia and well pronounced actin fibers were visible for
the MSCs from both sources. Moreover, cells were more spread after 24h in contact with {\textendash}
OSO3H containing surfaces. Cells behaved similarly on both sulfated surfaces (sHA and HEP)
and differences in cell morphology were less obvious: higher sulfation degree induced less
lamellipodia formation while filopodia number and length increased.
In summary, the present study provides evidence that sulfation degree of GAGs triggers
distinct cytoskeleton organisation in mesenchymal stem cells that may be related with the
differentiation of those cells. However, further studies at the molecular level about the exact
mechanism of these processes need to be carried out.

1. Capila I. and Linhardt R.J., Angewandte Chemie-International Edition, 2002.41(3):391-412.
2. Soares da Costa D. et al., Journal of Materials Chemistry, 2012.22(15):7172-7178.

Acknowledgement

This work was carried out under the scope of the EU project Find\&Bind-FP7/2007-2013 under
grant agreement no.NMP4-SL-2009-229292.

}, keywords = {Cytoskeleton, Glycosaminoglycans, mesenchymal stem cells, Sulfation}, doi = {10.1002/term.1608}, url = {http://onlinelibrary.wiley.com/doi/10.1002/term.1608/abstract}, author = {Soares da Costa, D. and Yang, Y. and Moller, S. and Groth, T. and Reis, R. L. and Pashkuleva, I.} }