@article {17467,
	title = {All-in-one rapid-prototyped bioreactor/implant for semi-automated generation of tailor-made critical size bone tissue substitutes},
	booktitle = {TERMIS World Congress},
	year = {2012},
	month = {2012-09-08 00:00:00},
	address = {Vienna, Austria},
	abstract = {<p><span>Additive Manufacturing (AM) offers the possibility to design and build\&nbsp;products with high geometrical \&nbsp;omplexity and in smaller batch numbers. In this work we applied AM to design and build a disposable bioreactor in combination with a scaffold. This work applies rapid\&nbsp;prototyping to design and fabricate scaffolds pre-enclosed into a disposable and ready-to-use perfusion chamber. Computerized tomography\&nbsp;was used to scan a 3 cm sheep tibia section and generate a porous\&nbsp;computer model replica. A fully enclosed perfusion chamber was\&nbsp;designed around the replica. As a proof of principle the perfusion\&nbsp;chamber and porous implant within were prototyped in one single\&nbsp;piece by fused deposition modeling of ABS. The device{\textquoteright}s watertightness\&nbsp;was confirmed by micro-CT and high flow perfusion studies. Micro-CT\&nbsp;also confirmed that although prototyped in one piece, one inside the\&nbsp;other, the implant and surrounding chamber were not merged together\&nbsp;at any point. Opening the chamber we observed that perfused fluids\&nbsp;had totally and homogenously reached the highly interconnected porous network of the scaffold. This device is currently being fabricated\&nbsp;from PLA for performing in vitro perfusion cell cultures in its interior.\&nbsp;This work demonstrated the possibility to produce easy- and ready-to-use fluid-efficient culture chambers pre-enclosing tailor-made and patient-specific scaffolds.</span></p>},
	keywords = {bioreactor, Rapid prototyping},
	author = {Costa, P. F. and Vaquette, C. and Gomes, M. E. and Reis, R. L. and Hutmacher, D. W.}
}
