@article {17415,
	title = {Surface microstructuring and protein patterning using hyaluronan derivatives},
	journal = {Microelectronic Engineering},
	volume = {106},
	year = {2013},
	month = {2013-02-08 00:00:00},
	pages = {21-26},
	publisher = {Elsevier},
	abstract = {<p>Natural polymers, such as hyaluronan, are considered as good candidates to substitute synthetic materials\&nbsp;in many biological applications due to their intrinsic biocompatibility for in vitro and living cell experiments.\&nbsp;This work describes surface modifications performed over modified hyaluronan derivatives\&nbsp;which could help to understand the physical and biochemical cues on the cell-behaviour. A photocrosslinkable\&nbsp;methacrylated hyaluronan was microstructured using soft lithography techniques to obtain a\&nbsp;microenvironment suitable for cell-behaviour experiments, which might mimic the extra-cellular matrix.\&nbsp;In addition, sulphated and non-sulphated oxidized hyaluronan were immobilized on bare substrates and\&nbsp;micrometric features of cell adhesive and non-adhesive proteins were patterned by microcontact printing\&nbsp;on top of them. The obtained structures were characterized by optical microscopy, profilometry and\&nbsp;atomic force microscopy. The stability of structures was tested by immersion in physiological salt solutions.\&nbsp;The observed results prove the suitability of the materials and protocols described.</p>},
	keywords = {Glycosaminoglycan, Hyaluronan, hydrogel, Microcontact printing, Microstructuring, Photocrosslinking},
	doi = {10.1016/j.mee.2013.01.039},
	url = {http://www.sciencedirect.com/science/article/pii/S0167931713000671},
	author = {M{\'a}rquez-Posadas, M. C. and Ramiro, J. and Becher, J. and Yang, Y. and K{\"o}witsch, A. and Pashkuleva, I. and D{\'\i}ez-Ahedo, R. and Schnabelrauch, M. and Reis, R. L. and Groth, T. and Merino, S.}
}
