@article {17385,
	title = {Liquidified capsules for cell immobilization coated with multilayered polyelectrolyte films},
	journal = { ADVANCED ENGINEERING MATERIALS},
	volume = {13},
	year = {2011},
	month = {2011-06-01 00:00:00},
	pages = {B218-B224},
	publisher = {John Wiley \& Sons},
	abstract = {<p>Natural-derived polymers are used to coat liquid-core capsules layer by layer to encapsulate cells. Human osteoblast-like cells (SaOs-2) are encapsulated in such spherical devices using a three-step methodology: i) ionotropic gelation to produce alginate beads encapsulating the cells; ii) layer-by-layer coating using water-soluble chitosan and alginate; and iii) core liquefaction. Cells remain viable for 3 d after the encapsulation procedure, suggesting that the developed capsules possess a semipermeable, nanostructured coating. All of the capsules exhibit a spherical shape, smooth surface and liquid-core characteristics. All of the processes are conducted under mild conditions and physiological pH. We consider that the methodology employed in the development of the capsules obtained from natural-based biomaterials has potential to find applicability in the development of scaffolds or cell carriers in tissue engineering and regenerative medicine.</p>},
	keywords = {ALGINATE, bioencapsulation, BIOMEDICAL APPLICATIONS, Chitosan, ENCAPSULATION TECHNOLOGY, L-LYSINE MICROCAPSULES, MICROENCAPSULATION, TRANSPLANTATION},
	doi = {10.1002/adem.201080138},
	url = {http://onlinelibrary.wiley.com/doi/10.1002/adem.201080138/abstract},
	author = {Costa, N. and Praveen, S. and Mano, J. F.}
}
