@article {17358, title = {Human Adipose Tissue-Derived SSEA-4 SubPopulation Multi-Differentiation Potential Towards the Endothelial and Osteogenic Lineages}, journal = {Tissue Engineering part A}, volume = {19}, year = {2012}, month = {2012-12-01 00:00:00}, pages = {235-246}, abstract = {

Human adipose tissue has been recently recognized as a potential source of stem cells for regenerative medicine applications, including bone tissue engineering (TE). Despite the gathered knowledge regarding the differentiation potential of human adipose tissue-derived stem cells (hASCs), in what concerns the endothelial lineage many uncertainties are still present. The existence of a cell subpopulation within the human adipose tissue that expresses a SSEA-4 marker, usually associated to pluripotency, raises expectations on the differ- entiation capacity of these cells (SSEA-4+ hASCs). In the present study, the endothelial and osteogenic dif- ferentiation potential of the SSEA-4+hASCs was analyzed, aiming at proposing a single-cell source/ subpopulation for the development of vascularized bone TE constructs. SSEA-4 + hASCs were isolated using immunomagnetic sorting and cultured either in a-MEM, in EGM-2 MV (endothelial growth medium), or in osteogenic medium. SSEA-4 + hASCs cultured in EGM-2 MV formed endothelial cell-like colonies character- ized by a cobblestone morphology and expression of CD31, CD34, CD105, and von Willebrand factor as determined by quantitative reverse transcriptase (RT)-polymerase chain reaction, immunofluorescence, and flow cytometry. The endothelial phenotype was also confirmed by their ability to incorporate acetylated low- density lipoprotein and to form capillary-like structures when seeded on Matrigel. SSEA-4 + hASCs cultured in a-MEM displayed fibroblastic-like morphology and exhibited a mesenchymal surface marker profile (\>90\% CD90+/CD73+/CD105+). After culture in osteogenic conditions, an overexpression of osteogenic-related markers (osteopontin and osteocalcin) was observed both at molecular and protein levels. Matrix minerali- zation detected by Alizarin Red staining confirmed SSEA-4+hASCs osteogenic differentiation. Herein, we demonstrate that from a single-cell source, human adipose tissue, and by selecting the appropriate subpop- ulation it is possible to obtain microvascular-like endothelial cells and osteoblasts, the most relevant cell types for the creation of vascularized bone tissue-engineered constructs.

}, keywords = {endothelial and osteogenic differentiation, SSEA-4+ human adipose derived stem/stromal cells}, doi = {10.1089/ten.tea.2012.0092}, url = {http://online.liebertpub.com/doi/abs/10.1089/ten.tea.2012.0092}, author = {Mihaila, S. M. and Frias, A. M. and Pirraco, R. P. and Rada, T. and Reis, R. L. and Gomes, M. E. and Marques, A. P.} }