@inproceedings {17257,
	title = {Evaluation oh the potential of Polyhydroxybutyrate-co-hydroxyvalerate bi-layered scaffolds for skin tissue engineering},
	journal = {Journal of Tissue Engineering and Regenerative Medicine},
	volume = {6},
	year = {2012},
	month = {2012-09-11 00:00:00},
	pages = {97-97},
	publisher = { John Wiley \& Sons},
	address = {3rd TERMIS World Congress 2012},
	abstract = {<p class="p1">Polyhydroxybutyrate-co-hydroxyvalerate (PHB-HV) is natural-based polyester produced by microorganisms under unbalanced growth con- ditions. This polymer is known for its biocompatibility and biodegrad- ability and has been explored for tissue engineering approaches. The aim of this work was to create a bi-layered construct, based on PHB- HV, for skin tissue replacement. The PHB-HV bi-layered scaffold was produced taking into consideration the two main skin strata, the epi- dermis and the dermis. For the epidermal fraction, a membrane was developed using a solvent cast approach, while freeze-drying was used to obtain the dermal fraction. The two structures were combined to obtain a scaffold that has a thin compact surface (epidermal) and a por- ous structure (dermis), as confirmed by scanning electron microscopy (SEM) and micro-CT analysis. Each layer of the scaffold, epidermal and dermal, was respectively seeded with human keratinocytes (Kc) and human dermal fibroblasts (Fb). The characterization by SEM con- firmed the typical morphology of Kc and Fb on the expected surfaces. Calcein-AM and DNA quantification showed that both type of cells remained viable along the experiment and were able to attach and pro- liferate on the respective seeding layers. In conclusion, the developed PHB-HV scaffolds have shown suitable properties to sustain the co-cul- ture of Kc and Fb, which constitutes a solid base for further work to demonstrate its potential for skin replacement.</p>},
	keywords = {natural-based polyester, Polyhydroxybutyrate-co-hydroxyvalerate},
	doi = {0.1002/term.1586},
	url = {http://onlinelibrary.wiley.com/doi/10.1002/term.1586/abstract},
	author = {Zonari, A. and Cerqueira, M. T. and Novikoff, S. and Goes, A. M. and Marques, A. P. and Correlo, V. M. and Reis, R. L.}
}
