@inproceedings {15891,
	title = {Preparation and characterization of macro/micro porous silk fibroin /nano-sized calcium phosphate scaffolds for bone tissue engineering},
	journal = {Journal of tissue engineering and regenerative medicine},
	volume = {6},
	year = {2012},
	month = {2012-09-30 00:00:00},
	pages = {181},
	publisher = {John Wiley \& Sons},
	address = {3rd TERMIS World Congress 2012, 5-8 September 2012, Vienna, Austria},
	abstract = {<p><br>Macro/micro porous silk scaffolds with nano-sized calcium phosphate (CaP) particles were developed for bone tissue engineering. Different amounts of nano-sized CaP particles 4, 8, 16 and 25\% (CaP:silk fibroin, wt/wt) were generated into the highly concentrated aqueous silk fibroin solutions via an in-situ approach. Afterwards, the pure silk and silk/nano-CaP porous scaffolds were produced by a combina- tion of salt-leaching/freeze-drying methods. Thermal gravimetric analysis results were able to demonstrated that the silk/nano-CaP scaffolds maintained 64{\textendash}87\% of incorporated CaP after salt-leaching. Dynamic mechanical analysis showed that storage modulus of the 16\% formulation was significantly higher than all remaining groups. The porosity of silk/nano-CaP scaffolds assessed using Micro-Com- puted Tomography decreased from 79.8\% to 63.6\% with increasing CaP incorporated until 16\%. By soaking the scaffolds in Simulated Body Fluid for 7 days, cauliflower-like apatite clusters were observed on the surface of both macro and micro pores of 16\% and 25\% for- mulations, which was not observed in 4 and 8\% formulations. 16\% silk/nano-CaP scaffolds were further chosen for in vitro cytotoxicity and biocompatibility assays. Both silk and 16\% silk/nano-CaP scaf- folds were non-cytotoxic and promoted cell adhesion and prolifera- tion to a similar extent. These results indicated that the 16\% silk/ nano-CaP scaffolds could be a good candidate for bone tissue engineering.</p>},
	keywords = {bone, Silk Fibroin},
	doi = {10.1002/term.1586},
	author = {Yan, L. P. and Correia, C. and Silva-Correia, J. and Caridade, S. G. and Fernandes, E. M. and Mano, J. F. and Sousa, R. A. and Oliveira, J. M. and Oliveira, A. L. and Reis, R. L.}
}
