@article {15831,
	title = {Understanding heat stress tolerance of suspended cells in the model plant Populus euphratica},
	journal = {ISRN Forestry},
	volume = {2012},
	year = {2012},
	month = {2012-06-07 00:00:00},
	pages = {1-5},
	publisher = {ISRN Forestry},
	abstract = {<p>A comprehensive understanding of the physiological responses of plants to extreme temperatures is essential for future strategies\&nbsp;for plant improvement. Obvious advantages can result from the study of highly adapted plant species, such as the model tree\&nbsp;Populus euphratica Olivier that naturally thrives under extreme temperatures, saline soils, and drought. The present paper addresses\&nbsp;the issue of P. euphratica thermotolerance using a cell suspension model system. P. euphratica suspended cells were subjected to a<br>range of temperatures (from 5 up to 75{\textopenbullet}C) for 20 min, and cultures were evaluated for cell viability and biomass content at specific\&nbsp;time points. The results have shown that cell viability was only affected after a temperature stress higher than 40{\textopenbullet}C, although in\&nbsp;these conditions it was observed that a cell growth increases after the recovery period. In contrast, a total decline in cell viability\&nbsp;was observed in suspended cells treated at 50{\textopenbullet}C or higher temperatures, which did not show growth recovery capacity. Therefore,\&nbsp;the known natural tolerance of P. euphratica to thermal stress was not observable at the cellular level. The greater susceptibility to\&nbsp;high temperatures in suspended cells as compared to field plants suggests that high thermotolerance can only be achieved when\&nbsp;cells are integrated into a tissue.</p>},
	keywords = {oxidative stress, Populus euphratica},
	doi = {10.5402/2012/243694},
	url = {http://www.hindawi.com/isrn/forestry/2012/243694/},
	author = {Silva-Correia, J. and Azevedo, H. S. and Lino-Neto, T. and Tavares, R. M.}
}
