@article {15755, title = {Chemical modification of bioinspired superhydrophobic polystyrene surfaces to control cell attachment/proliferation}, journal = {Soft Matter}, volume = {7}, year = {2011}, month = {2011-09-21 00:00:00}, pages = {8932-8941}, publisher = {RSC}, abstract = {

Surface chemistry is known to have a profound influence on cellular behavior. However, thecells{\textquoteright} responses on surfaces exhibiting extreme wettability values are not yet fully understood. In this work, bioinspired superhydrophobic\ polystyrene\ (PS) surfaces were produced by a phase\ separation method\ and the\ in vitro\ performance of three different\ cell\ lines (SaOs-2, L929 and\ ATDC5) was assessed on those surfaces. Compared to standard tissue culture\ PS, it was found that\ ATDC5\ and\ SaOs-2\ cell\ lines were not able to proliferate on such surfaces and the\ cell\ morphology was affected; however\ L929\ cells\ could proliferate. The attachment, morphology and proliferation of\ SaOs-2\ were studied on rough and smooth\ PS\ surfaces with wettability controlled by UV/O3\ (UVO) irradiation, ranging from superhydrophobic to superhydrophilic. After 4 hours in culture, the attachment of\ SaOs-2\ was higher on the surfaces treated during 18 minutes, namely on rough superhydrophilic and highly hydrophilic smooth\ PSsurfaces. The proliferation after 6 days in culture was higher in surfaces with\ water\ contact angles ranging from 13{\textdegree} to 30{\textdegree}, independently of being rough or smooth. Moreover, well defined superhydrophilic spots were patterned on superhydrophobic surfaces, by using hollowed masks to localize the\ UVO\ irradiation treatment. It was possible to localize the proliferation ofSaOs-2\ cells\ on these superhydrophilic spots. This work suggests that the introduction of random micro/nano roughness and further chemical modification by\ UVO\ irradiation may be an elegant and easy method to control spatially the attachment/proliferation of\ cells\ in distinct materials with possible uses in high-throughput analysis, microfluidic systems or even in 3D systems.

}, keywords = {patterning, superhydrophobic}, url = {http://pubs.rsc.org/en/Content/ArticleLanding/2011/SM/c1sm05943b}, author = {Oliveira, S. M. and Song, W. and Alves, N. M. and Mano, J. F.} }