@article {15723, title = {Use of animal protein-free products for passaging adherent human adipose-derived stromal/stem cells.}, journal = {Cytotherapy}, volume = {13}, year = {2011}, month = {2011-05-02 00:00:00}, pages = {594-7}, abstract = {

Adherent adipose-derived stromal/stem cells (ASC) have been used in pre-clinical regenerative medical studies applied to
a broad range of tissues with an ultimate goal of translating these fi ndings to clinical safety and effi cacy testing; however,
many protocols passage the cells using porcine-derived trypsin. We have compared porcine trypsin with animal protein-free
products from recombinant bacteria (TrypLE Express; Invitrogene) and corn (TrypZean; Sigma) based on cell yield,
viability and immunophenotype. ASC harvested with each trypsin product were comparable.

}, keywords = {protein-free products, TE}, author = {Carvalho, P. P. and Wu, X. and Yu, G. and Dietrich, M. and Dias, I. R. and Gomes, M. E. and Reis, R. L. and Gimble, J. M.} }